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Towards on-line monitoring of cell growth in microporous scaffolds: Utilization and interpretation of complex permittivity measurements.

TitleTowards on-line monitoring of cell growth in microporous scaffolds: Utilization and interpretation of complex permittivity measurements.
Publication TypeJournal Article
Year of Publication2003
AuthorsBagnaninchi PO, Dikeakos M, Veres T, Tabrizian M
JournalBiotechnol Bioeng
Volume84
Issue3
Pagination343-50
Date Published2003 Nov 5
ISSN0006-3592
KeywordsAlgorithms, Animals, Cell Count, Cell Division, Chitin, Chitosan, Computer Simulation, Culture Techniques, Electric Capacitance, Electric Impedance, Electrochemistry, Extracellular Matrix, Macrophages, Membranes, Artificial, Mice, Models, Biological, Online Systems, Reproducibility of Results, Sensitivity and Specificity, Tissue Engineering
Abstract

Here we demonstrate the ability to characterize microporous scaffolds and evaluate cell concentration variation via the utilization and interpretation of complex permittivity measurements (CP), a direct and nondestructive method. Polymer-based microporous scaffolds are of importance to tissue engineering, particularly in the promotion of cell adhesion, proliferation, and differentiation in predefined shapes. Chitosan gel scaffolds were seeded with increasing concentrations of macrophages to simulate cell growth. Complex permittivity measurements were performed using a dielectric probe and a vector network analyzer over a frequency ranging from 200 MHz to 2 GHz. An effective medium theory was applied to interpret the data obtained; respectively, Looyenga and Maxwell-Wagner-Hanai functions were used to retrieve the porosity and the variation of the cell concentration from the CP measurements. Calculated porosities were in agreement with experimental evaluation-porosity ranged from 81-96%. Changes in cell concentration inside the scaffolds upon injection of differing cell concentrations into the scaffold were detected distinguishably. Variations resulting from the cumulative injection of 400-1800 microL of 10(6) cells/mL solution into the scaffold were monitored. Results suggest that CP measurements in combination with an appropriate effective medium approximation can enable on-line monitoring of cell growth within scaffolds.

DOI10.1002/bit.10770
Alternate JournalBiotechnol. Bioeng.
PubMed ID12968288
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