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Targeted SERS nanosensors measure physicochemical gradients and free energy changes in live 3D tumor spheroids.

TitleTargeted SERS nanosensors measure physicochemical gradients and free energy changes in live 3D tumor spheroids.
Publication TypeJournal Article
Year of Publication2016
AuthorsJamieson LE, Camus VL, Bagnaninchi PO, Fisher KM, Stewart GD, Nailon WH, McLaren DB, Harrison DJ, Campbell CJ
JournalNanoscale
Volume8
Issue37
Pagination16710-16718
Date Published2016 Sep 22
ISSN2040-3372
Abstract

Use of multicellular tumor spheroids (MTS) to investigate therapies has gained impetus because they have potential to mimic factors including zonation, hypoxia and drug-resistance. However, analysis remains difficult and often destroys 3D integrity. Here we report an optical technique using targeted nanosensors that allows in situ 3D mapping of redox potential gradients whilst retaining MTS morphology and function. The magnitude of the redox potential gradient can be quantified as a free energy difference (ΔG) and used as a measurement of MTS viability. We found that by delivering different doses of radiotherapy to MTS we could correlate loss of ΔG with increasing therapeutic dose. In addition, we found that resistance to drug therapy was indicated by an increase in ΔG. This robust and reproducible technique allows interrogation of an in vitro tumor-model's bioenergetic response to therapy, indicating its potential as a tool for therapy development.

DOI10.1039/c6nr06031e
Alternate JournalNanoscale
PubMed ID27714168
Publication institute
CRM