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Quantitative functions of Argonaute proteins in mammalian development.

TitleQuantitative functions of Argonaute proteins in mammalian development.
Publication TypeJournal Article
Year of Publication2012
AuthorsWang D, Zhang Z, O'Loughlin E, Lee T, Houel S, O'Carroll D, Tarakhovsky A, Ahn NG, Yi R
JournalGenes Dev
Volume26
Issue7
Pagination693-704
Date Published2012 Apr 1
ISSN1549-5477
KeywordsAnimals, Argonaute Proteins, Cell Proliferation, Eukaryotic Initiation Factors, Gene Expression Regulation, Developmental, Melanoma, Mice, Mice, Knockout, MicroRNAs, Skin
Abstract

Argonaute proteins (Ago1-4) are essential components of the microRNA-induced silencing complex and play important roles in both microRNA biogenesis and function. Although Ago2 is the only one with the slicer activity, it is not clear whether the slicer activity is a universally critical determinant for Ago2's function in mammals. Furthermore, functional specificities associated with different Argonautes remain elusive. Here we report that microRNAs are randomly sorted to individual Argonautes in mammals, independent of the slicer activity. When both Ago1 and Ago2, but not either Ago1 or Ago2 alone, are ablated in the skin, the global expression of microRNAs is significantly compromised and it causes severe defects in skin morphogenesis. Surprisingly, Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression. Quantitative analyses reveal that Ago2 interacts with a majority of microRNAs (60%) in the skin, compared with Ago1 (30%) and Ago3 (

DOI10.1101/gad.182758.111
Alternate JournalGenes Dev.
PubMed ID22474261
PubMed Central IDPMC3323880
Grant ListR00 AR054704 / AR / NIAMS NIH HHS / United States
R00 AR054704-05 / AR / NIAMS NIH HHS / United States
R00AR054704 / AR / NIAMS NIH HHS / United States
R01 AR059697 / AR / NIAMS NIH HHS / United States
R01 AR059697-02 / AR / NIAMS NIH HHS / United States
R01AR059697 / AR / NIAMS NIH HHS / United States
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