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Maternal microRNAs are essential for mouse zygotic development.

TitleMaternal microRNAs are essential for mouse zygotic development.
Publication TypeJournal Article
Year of Publication2007
AuthorsTang F, Kaneda M, O'Carroll D, Hajkova P, Barton SC, Y Sun A, Lee C, Tarakhovsky A, Lao K, M Surani A
JournalGenes Dev
Volume21
Issue6
Pagination644-8
Date Published2007 Mar 15
ISSN0890-9369
KeywordsAnimals, Embryonic Development, Female, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Knockout, MicroRNAs, Oocytes, Pregnancy, Ribonuclease III, Zygote
Abstract

MicroRNAs (miRNAs) have important roles in diverse cellular processes, but little is known about their identity and functions during early mammalian development. Here, we show the effects of the loss of maternal inheritance of miRNAs following specific deletion of Dicer from growing oocytes. The mutant mature oocytes were almost entirely depleted of all miRNAs, and they failed to progress through the first cell division, probably because of disorganized spindle formation. By comparing single-cell cDNA microarray profiles of control and mutant oocytes, our data are compatible with the notion that a large proportion of the maternal genes are directly or indirectly under the control of miRNAs, which demonstrates that the maternal miRNAs are essential for the earliest stages of mouse embryonic development.

DOI10.1101/gad.418707
Alternate JournalGenes Dev.
PubMed ID17369397
PubMed Central IDPMC1820938
Grant List / / Wellcome Trust / United Kingdom
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