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A Highly Responsive System for On-line in vitro Assessment of Tissue Growth within MicroPorous Polymer Scaffolds.

TitleA Highly Responsive System for On-line in vitro Assessment of Tissue Growth within MicroPorous Polymer Scaffolds.
Publication TypeJournal Article
Year of Publication2005
AuthorsDziong D, Kearney RE, Tabrizian M, Bagnaninchi PO
JournalConf Proc IEEE Eng Med Biol Soc
Volume1
Pagination1043-6
Date Published2005
ISSN1557-170X
Abstract

We have proposed a highly responsive system for the on-line in vitro assessment of tissue growth within microporous polymer scaffolds that obviates any compromise of sample integrity. The system's function is based on the sample's loss factor: the imaginary part of the complex permittivity. Reflection measurements were performed using an open-ended coaxial probe and impedance analyzer; they were then related to the sample's complex permittivity by a quasi-static model of the probe's aperture admittance. Measurements of saline solutions showed that the real part of permittivity was corrupted by apparent polarization effects. Consequently, we developed a simplified formulation of the imaginary part of the Hanai-Wagner effective medium approximation to eliminate its dependence on the real part of complex permittivity measurement. This formulation allows the sample's cell concentration to be determined. The variation of a sample's cell concentration over time was used as a measure of tissue growth. Measurements in the frequency range of 10-200 MHz were performed on micro-porous polymer scaffolds seeded with progressively greater number of cells. Results demonstrated that the system detected concentration differences between cell-seeded scaffolds.

DOI10.1109/IEMBS.2005.1616597
Alternate JournalConf Proc IEEE Eng Med Biol Soc
PubMed ID17282366
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