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Formation of embryoid bodies using dielectrophoresis.

TitleFormation of embryoid bodies using dielectrophoresis.
Publication TypeJournal Article
Year of Publication2012
AuthorsAgarwal S, Sebastian A, Forrester LM, Markx GH
JournalBiomicrofluidics
Volume6
Issue2
Pagination24101-2410111
Date Published2012 Jun
ISSN1932-1058
Abstract

Embryoid body (EB) formation forms an important step in embryonic stem cell differentiation invivo. In murine embryonic stem cell (mESC) cultures EB formation is inhibited by the inclusion of leukaemic inhibitory factor (LIF) in the medium. Assembly of mESCs into aggregates by positive dielectrophoresis (DEP) in high field regions between interdigitated oppositely castellated electrodes was found to initiate EB formation. Embryoid body formation in aggregates formed with DEP occurred at a more rapid rate-in fact faster compared to conventional methods-in medium without LIF. However, EB formation also occurred in medium in which LIF was present when the cells were aggregated with DEP. The optimum characteristic size for the electrodes for EB formation with DEP was found to be 75-100 microns; aggregates smaller than this tended to merge, whilst aggregates larger than this tended to split to form multiple EBs. Experiments with ESCs in which green fluorescent protein (GFP) production was targeted to the mesodermal gene brachyury indicated that differentiation within embryoid bodies of this size may preferentially occur along the mesoderm lineage. As hematopoietic lineages during normal development derive from mesoderm, the finding points to a possible application of DEP formed EBs in the production of blood-based products from ESCs.

DOI10.1063/1.3699969
Alternate JournalBiomicrofluidics
PubMed ID22655013
PubMed Central IDPMC3360717