Dr Gareth A. Roberts

Position:

Senior PostDoc

g.a.roberts@ed.ac.uk

Biography:

My research focuses on engineering synthetic transcription factors for cellular reprogramming applications.

Publications

2014

ArdA proteins from different mobile genetic elements can bind to the EcoKI Type I DNA methyltransferase of E. coli K12. Chen, Kai, Reuter Marcel, Sanghvi Bansi, Roberts Gareth A., Cooper Laurie P., Tilling Matthew, Blakely Garry W., and Dryden David T. F. , Biochim Biophys Acta, 2014 Mar, Volume 1844, Issue 3, p.505-11, (2014)

2013

DNA electrophoresis: historical and theoretical perspectives. Roberts, Gareth A., and Dryden David T. F. , Methods Mol Biol, 2013, Volume 1054, p.1-9, (2013)

Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerization and antimodification activity but not antirestriction activity. Roberts, Gareth A., Chen Kai, Bower Edward K. M., Madrzak Julia, Woods Arcadia, Barker Amy M., Cooper Laurie P., White John H., Blakely Garry W., Manfield Iain, et al. , FEBS J, 2013 Oct, Volume 280, Issue 19, p.4903-14, (2013)

Impact of target site distribution for Type I restriction enzymes on the evolution of methicillin-resistant Staphylococcus aureus (MRSA) populations. Roberts, Gareth A., Houston Patrick J., White John H., Chen Kai, Stephanou Augoustinos S., Cooper Laurie P., Dryden David T. F., and Lindsay Jodi A. , Nucleic Acids Res, 2013 Aug, Volume 41, Issue 15, p.7472-84, (2013)

2012

Removal of a frameshift between the hsdM and hsdS genes of the EcoKI Type IA DNA restriction and modification system produces a new type of system and links the different families of Type I systems. Roberts, Gareth A., Chen Kai, Cooper Laurie P., White John H., Blakely Garry W., and Dryden David T. F. , Nucleic Acids Res, 2012 Nov, Volume 40, Issue 21, p.10916-24, (2012)

Exploring the DNA mimicry of the Ocr protein of phage T7. Roberts, Gareth A., Stephanou Augoustinos S., Kanwar Nisha, Dawson Angela, Cooper Laurie P., Chen Kai, Nutley Margaret, Cooper Alan, Blakely Garry W., and Dryden David T. F. , Nucleic Acids Res, 2012 Sep, Volume 40, Issue 16, p.8129-43, (2012)

Structure and operation of the DNA-translocating type I DNA restriction enzymes. Kennaway, Christopher K., Taylor James E., Song Chun Feng, Potrzebowski Wojciech, Nicholson William, White John H., Swiderska Anna, Obarska-Kosinska Agnieszka, Callow Philip, Cooper Laurie P., et al. , Genes Dev, 2012 Jan 1, Volume 26, Issue 1, p.92-104, (2012)

2011

An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme. Roberts, Gareth A., Cooper Laurie P., White John H., Su Tsueu-Ju, Zipprich Jakob T., Geary Paul, Kennedy Cowan, and Dryden David T. F. , Nucleic Acids Res, 2011 Sep 1, Volume 39, Issue 17, p.7667-76, (2011)

2010

Fusion of GFP to the M.EcoKI DNA methyltransferase produces a new probe of Type I DNA restriction and modification enzymes. Chen, Kai, Roberts Gareth A., Stephanou Augoustinos S., Cooper Laurie P., White John H., and Dryden David T. F. , Biochem Biophys Res Commun, 2010 Jul 23, Volume 398, Issue 2, p.254-9, (2010)

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro. Serfiotis-Mitsa, Dimitra, Herbert Andrew P., Roberts Gareth A., Soares Dinesh C., White John H., Blakely Garry W., Uhrín Dusan, and Dryden David T. F. , Nucleic Acids Res, 2010 Mar, Volume 38, Issue 5, p.1723-37, (2010)

2009

Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme. Robin, Aélig, Roberts Gareth A., Kisch Johannes, Sabbadin Federico, Grogan Gideon, Bruce Neil, Turner Nicholas J., and Flitsch Sabine L. , Chem Commun (Camb), 2009 May 14, Issue 18, p.2478-80, (2009)

Dissection of the DNA mimicry of the bacteriophage T7 Ocr protein using chemical modification. Stephanou, Augoustinos S., Roberts Gareth A., Cooper Laurie P., Clarke David J., Thomson Andrew R., C MacKay Logan, Nutley Margaret, Cooper Alan, and Dryden David T. F. , J Mol Biol, 2009 Aug 21, Volume 391, Issue 3, p.565-76, (2009)

Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance. McMahon, Stephen A., Roberts Gareth A., Johnson Kenneth A., Cooper Laurie P., Liu Huanting, White John H., Carter Lester G., Sanghvi Bansi, Oke Muse, Walkinshaw Malcolm D., et al. , Nucleic Acids Res, 2009 Aug, Volume 37, Issue 15, p.4887-97, (2009)

A mutational analysis of DNA mimicry by ocr, the gene 0.3 antirestriction protein of bacteriophage T7. Stephanou, Augoustinos S., Roberts Gareth A., Tock Mark R., Pritchard Emily H., Turkington Rachel, Nutley Margaret, Cooper Alan, and Dryden David T. F. , Biochem Biophys Res Commun, 2009 Jan 2, Volume 378, Issue 1, p.129-32, (2009)

2008

The Orf18 gene product from conjugative transposon Tn916 is an ArdA antirestriction protein that inhibits type I DNA restriction-modification systems. Serfiotis-Mitsa, Dimitra, Roberts Gareth A., Cooper Laurie P., White John H., Nutley Margaret, Cooper Alan, Blakely Garry W., and Dryden David T. F. , J Mol Biol, 2008 Nov 28, Volume 383, Issue 5, p.970-81, (2008)

Search form