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Leica TCS SP8 Confocal and CW-STED Nanoscopy Microscope

Leica TCS SP8 Confocal and CW-STED Nanoscopy Microscope

Introduction

Location: SCRM ground floor, room G.43

Access (after training): 24/24h, 7/7 days

Cost (CRM users): £44 peak hours (8am-5pm, week days), £33 off-peak, time-lapse session £200

Specifications

Main features:

  • Inverted microscope

  • Lasers: 405, 458/476/488/496/514, 561, 594, 633 nm

  • Filter-free spectral detection 400-800 nm

  • Fast resonant scanner (12kHz)

  • Super-resolution with 592 nm CW depletion laser

  • 2 HyD detectors (45% QE @ 500 nm) and 3 PMT (30% QE @ 500 nm) for fluorescence imaging, 1 PMT for transmitted light

  • Fully motorised XY stage for multi-positions, tilling, …

  • galvanometer stage for Z-focus (range 1500 µm, minimum step size 20 nm)

  • Environmental chamber (temperature and 5% CO2)

  • Software-controlled water pump for water immersion objectives

  • Adaptive Focus Control (AFC) to correct for focus drift

  • Advanced imaging techniques: FRAP, FRET, photo-activation, time-lapse, high-content imaging (MatrixScreener module)

Objectives (More objectives available upon request at the time of booking)

  • 10x, NA 0.40, Air
  • 20x, NA 0.7, multi-immersion (Water, Glycerol, Oil)
  • 40x, NA 1.25, Oil
  • 63x, NA 1.4, Oil
  • 25x, NA 0.95, Water (automatic water immersion)
  • 40x, NA 1.1, Water (automatic water immersion)

Sample carriers:

Slides or chambered-slides

Petri dishes (35 mm, 58 mm)

 Multi-well plates

Contact