This service offers ES cell line derivation directly from blastocysts from wild type and mutant mice strains. This technique is highly efficient and utilising serum free media containing small molecule inhibitors (2i media).

We offer:

• Immunosurgery and explant culture in 2i serum free media
• Expansion, passage and freezing of new ES cell lines in 2i media
• Conversion of 2i derived ES cells into standard serum containing media
• DNA preparation for screening of derived lines
• Mycoplasma testing of derived lines
• Distribution of early passage, germline-tested ES cells

We can derive ES cells from a wide variety of mouse strains including 129Sv and C57Bl/6. Please contact the Transgenic Service to discuss your exact needs.