The Transgenic Service offers services to aid gene targeting in ES cells as well as microinjection of targeted lines for germline transmission.

We offer:

• Electroporation of DNA constructs into validated germline-competent ES cells as well as selection, expansion and freezing of targeted clones. Chromosomal DNA is returned for analysis of targeting events. We subsequently expand positive cell lines, prepare DNA for confirmation to prepare clones for microinjection.  Currently the service does not offer construction of targeting vectors or Southern blot screening of clones. However, our experience team can provide support and advice for this.
• Microinjection of targeted clones. Chimeric mouse production and testing for germline via coat colour transmission. This service is available to other academic facilities and companies on a recharge basis. Please be aware that ES cells targeted in other facilities will require pathogen testing prior to injection for an additional charge.